Where in the oviduct does fertilization of the ovulated yolk by rooster spermatozoa take place?

Fertilization in chickens is a highly time-sensitive process localized in the infundibulum, the topmost, funnel-shaped segment of the oviduct. Following mating, spermatozoa swim up the entire length of the oviduct and are stored in specialized sperm host glands at the utero-vaginal junction, before migrating to secondary storage glands in the infundibulum. When ovulation occurs, the mature yolk (ovum) is released from the ovary and captured by the infundibulum funnel. The spermatozoa must penetrate the vitelline membrane of the yolk at the germinal disc within a narrow 15-minute window. Once the yolk enters the magnum, the secretion of the thick albumen layers begins, forming a physical barrier that prevents any further sperm entry. Breeder managers can study fertility biology in the Poultry Encyclopedia, buy high-fertility rooster feeds on Poultry Plaza, track weekly chick and hatching egg rates on Poultry Rates, and buy/sell hatching eggs on Murghi Mandi.